Aqueous solutions are stable for at. The hoechst BD Pharmingen™ Hoechst 33342 Solution is a reagent for the fluorescent staining of DNA nuclei in live fixed cells. hoechst This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for hoechst cell cycle studies in combination with BrdU. HoechstNone None None None EU OEL ( STEL) EU Skin Notation Chemical Name Austria Denmark Poland Switzerland HoechstNone HoechstNone None None None None None Handling Chemical Name Ireland Norway Spain OEL ( TWA) HoechstNone None None Avoid contact with skin eyes clothing. datasheet Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double- stranded AT- rich regions. Hoechst- 33342 unlike other Hoechst dyes is more lipophillic due to an. 1 It emits blue fluorescence ( excitation 350 nm/ emission maximum 461 nm) when bound to double stranded DNA is useful as datasheet a marker of nuclei for cell cycle studies to distinguish nuclear morphology in.
Storage: The lyophilized powder is stable for 1 year if kept below 0ºC with desiccant. Replicating or dividing chromosomes will not stain with Hoechst 33342 DNA dye. For batch specific data refer to the Certificate of Analysis. This product may be used in fluorescence microscopy , microplate, cuvette flow cytometry applications. A comparative footprinting study. Description Hoechst 33342 trihydrochloride is a membrane permeant hoechst blue fluorescent DNA stain. The technical data provided above is for guidance only. Excitation of the dye occurs around 350 nm with an emission maxima around datasheet 461 datasheet nm.
hoechst Hoechst 33342 datasheet Staining Dye datasheet Solution ( ab228551) is a fluorescent stain for labeling DNA in fluorescence microscopy. The compound is excited by ultraviolet light at around 350 nm emits blue/ cyan fluorescence light around an emission maximum at 461 nm. Hoechst 33342 nucleic acid stain is a popular cell- permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. Biochim Biophys Acta 949, 158- 68. IC₅₀ & Target Dye reagent[ hoechst 1] DNA Stain[ 1] In Vitro Hoechst 33342 binds to adenine- thymine- rich regions of DNA in the minor groove. Hoechst 33342 trihydrochloride is an A: T- hoechst specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. 93) that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of. Hoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double- stranded DNA ( preferentially binds to A- T base pairs). datasheet hoechst On binding to DNA, the fluorescence greatly increases. Portugal and datasheet Waring Biochim.
93) that is able to stain the hoechst DNA of eukaryotic and prokaryotic cells by datasheet binding with high affinity to the minor groove of AT- rich DNA sequences. Hoechst 33342 is a cell- permeable fluorescent compound ( MW 561. Thermo Scientific Pierce Hoechst 33342 datasheet Fluorescent Stain is a high- quality solution of Hoechst dye for fixed- live- cell fluorescent staining of DNA nuclei in cellular imaging techniques. Assignment of DNA binding sites for 4' 6- diamidine- 2- phenylindole bisbenzimide ( Hoechst 33258).
Hoechst 33342 Staining Dye Solution ( ab228551) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be. datasheet on our website. Hoechst 33342 emits blue ﬂ uorescence when bound to double stranded DNA.
hoechst 33342 datasheet
It is slightly more membrane per- meant than the Hoechst 33258 analog. Hoechst 33342 may be used to identify healthy or apoptotic nuclear mor- phology and for cell cycle studies. Each vial of Hoechst 33342 contains 1 mL of aqueous solution at 200 μg/ mL ( catalog # 639).